Nature | IMC Uncovering the single-cell spatial landscape of the immune microenvironment in lung cancer

Single-cell technologies reveal the complexity of the tumour immune microenvironment with precise resolution. Most clinical diagnoses rely on histopathological stratification of tumour subtypes, but little is known about the spatial mapping of single-cell phenotypes within these stratified subgroups. Recently, Logan Walsh's team at the Goodman Cancer Institute at McGill University in Canada published a study in Nature titled "Single-cell spatial landscapes of the lung tumour immune microenvironment ". The study characterised the immune microenvironment of 416 non-small cell lung cancer samples by imaging mass spectrometry flow-through (IMC), deeply resolved 1.6 million cells, and analysed the correlation between cellular immune profiles and activation status and clinical outcomes of patients.

2023-05-03

Nature Communications | Flip through the scRNA-seq dataset and add some IMC data analysis to give you a different high score experience!

Tumour-associated fibroblasts (CAF) are a diverse population of cells in the tumour microenvironment that have a significant impact on tumour evolution and patient prognosis.
To define CAF phenotypes, the researchers analysed a single-cell RNA sequencing (scRNA-seq) dataset of more than 16,000 mesenchymal stromal cells from the tumours of 14 breast cancer patients, on the basis of which nine CAF phenotypes and a class of pericytes were defined and functionally annotated. The researchers validated this classification system in four additional cancer types and used tissue imaging mass spectrometry flow (IMC) technology on matched breast cancer samples to confirm CAF phenotypes defined at the protein level and to analyse their spatial distribution in the tumour. This CAF classification scheme will allow comparison of CAF phenotypes from different studies, facilitate analysis of their functional role and potentially guide the development of new therapeutic strategies in the future.

2023-11-30

IF=24.3: scRNA-seq & mIHC uncover mechanisms of drug resistance in gastric cancer

Anti-PD1+ chemotherapy is an integral part of the comprehensive treatment of gastric cancer (GC). In patients with progressive gastric cancer, the use of neoadjuvant immunotherapy plus chemotherapy elicits sustained responses, but the majority of patients exhibit primary resistance and some of those who do respond often develop acquired resistance over time. There is an urgent need to study the cellular and molecular basis of drug resistance in this context.

2024-04-10

Clinical Trial Programmes | CODEX reveals microenvironmental ecology of TLSs in chronic blisters of pemphigus

Pemphigus is a rare life-threatening autoimmune disease that presents as blisters and erosions on the skin and/or mucous membranes. Although tertiary lymphoid structures (TLSs ) have been detected in the inflammatory lesions of patients with pemphigus, the role of TLSs in the pathogenesis of pemphigus remains unclear.

Recently, researchers from Yonsei University College of Medicine published a paper titled "Microenvironmental network of clonal CXCL13+CD4+ T cells and regulatory T cells in pemphigus" in the Journal of Clinical Investigation (IF=15.9). cells and regulatory T cells in pemphigus chronic blisters" in the Journal of Clinical Investigation (IF=15.9), revealing the presence of TLSs containing DSG-specific B cells and plasma cells and the up-regulation of CXCL13 expression in chronic blisters of pemphigus patients.

2023-12-14