Application Cases

• Circ Res | IMC maps single-cell protein modifications in spatiotemporal mapping of myocardial ischaemia-reperfusion injury in mice

  Professor Wang Li's team at the State Key Laboratory of Cardiovascular Diseases, Fu Wai Hospital, Chinese Academy of Medical Sciences, used IMC to map the spatio-temporal structure and post-translational modifications of mouse cardiac cellular structure after IRI with single-cell resolution and found that H3K9me3 in the endothelial cells is an important potential target for the treatment of cardiac pathological remodelling after IRI.

  
• Nature | IMC Uncovering the single-cell spatial landscape of the lung cancer immune microenvironment

  Single-cell technologies reveal the complexity of the tumour immune microenvironment with precise resolution. Most clinical diagnoses rely on histopathological stratification of tumour subtypes, but little is known about the spatial mapping of single-cell phenotypes in these stratified subgroups. This study characterised the immune microenvironment of 416 non-small cell lung cancer samples by IMC, resolved 1.6 million cells in depth, and analysed the correlation between cellular immune profiles and activation status and patient clinical outcomes.

  
• Nature | IMC Uncovering the single-cell spatial immune landscape of primary and metastatic brain tumours

  The study used IMC to describe the tumour microenvironment in 139 patients with high-grade glioma (Glioma) and 46 patients with brain metastases (BrM), revealing differences in the immune landscape between primary tumours and brain metastases of different solid carcinomas through single-cell analyses of more than 1.1 million cells in 389 high-dimensional histopathological images, as well as identifying, in glioblastoma patients, an association with amyeloperoxidase (MPO)-positive macrophage population associated with long-term survival. The findings provide insights into the biology of primary and metastatic brain tumours, reinforcing the value of integrating spatial resolution into single-cell datasets to dissect the microenvironment of cancer.

  
• Nature | IMC predicts immune efficacy in triple-negative breast cancer

  By mapping multicellular tumour ecosystems in situ in TNBC with IMC technology, the researchers identified key predictors of the ICB response and showed that ICB uniquely remodels tumour architecture.The application of IMC technology in this article demonstrates that the systematic mapping of complete tumour ecosystems can enable precise immuno-oncology.