High-throughput single-cell immunohistochemistry libraries are used to rapidly complete 5' transcriptome library construction and TCR & BCR library construction in up to tens of thousands of single cells. Kits include molecularly labeled microspheres for single-cell nucleic acid capture, microfluidic microarrays, reverse transcription amplification reagents, transcriptome library amplification reagents, and TCR & BCR library amplification reagents to meet the needs of 5' transcriptome library construction and TCR & BCR library construction.